Our course curriculum will cover the following topics:

Introduction
Components of a flow cytometer -
     fluidics, optics, electronics
Instrument Characterization
Instrument Demonstration
Applications

      Variations on the Immunophenotyping
         Intracellular Protein detection
         Other special applications
         Functional Assays
         DNA analysis - Introduction
         Molecular cytometry
               End of Day 1

Designing Good Assays
      Choosing Antibodies / Fluorochromes
      Using Fluorochromes and Reagents
Staining and Controls
Compensation
       How to do it correctly
        Measurement Error
        Hardware vs Software
        Bi-Exponential Scaling
Introduction to  Flow Cytometric Data
     how data is generated
     linear/log, transforms, binning / channels
Data Display and Gating
     Gating strategies -  live and post acquisition
        Multicolor with emphasis on how to analyze and interpret

        uni- and bi-variant histograms

Resources

Protocols, Bibliographies,  and Information Sources

Where to go from here

Other courses, tutorials, meetings, vendor offerings

Please note: This training is not instrument specific, but applicable to any and all instruments.  We strive to teach you how to get the most out of your cytometer, not how to push the buttons. 

Typical training times are  9:00 AM to 5:00 PM each day for 2 days and most often offered as the first 2 days of our Comprehensive.  This course is most appropriate for novices planning on only 2-3 color flow.  The 3 Day comprehensive is suggested for everyone using a multiparameter instrument.