Our course curriculum will cover the following topics:
 

Introduction
Components of a flow cytometer -
     fluidics, optics, electronics
Instrument Characterization
Instrument Demonstration
Applications

      Variations on the Immunophenotyping
         Intracellular Protein detection
         Other special applications
         Functional Assays
         DNA analysis - Introduction
         Molecular cytometry
               End of Day 1

Designing Good Assays
      Choosing Antibodies / Fluorochromes
      Using Fluorochromes and Reagents
Staining and Controls
Compensation
       How to do it correctly
        Measurement Error
        Hardware vs Software
        Bi-Exponential Scaling
Introduction to  Flow Cytometric Data
     how data is generated
     linear/log, transforms, binning / channels
Data Display and Gating
     Gating strategies -  live and post acquisition
        Multicolor with emphasis on how to analyze and interpret

        uni- and bi-variant histograms
                    End of Day 2

Statistics
Presenting and Reporting Your Data
     Putting the data altogether
Data Demonstration
Strategies for Building Multicolor Panels
     Design Process
        Antibody / Fluorochrome combinations
        Necessary, Important or Luxury Markers
        Calculating Spread and error
        Instrument configurations / filter choices
Practice Designing Experiments
     Biological hypothesis
     Trying some of these examples
Future of Data Presentation and Analysis
Troubleshooting
                End of Day 3

Manual also includes
    Resources
    Protocols, Bibliographies,  and Information Sources
    Where to go from here
    Other courses, tutorials, meetings, vendor offerings