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Session I |
Introduction |
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what
is it, not how it works.... yet |
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Session II |
Principals of
Instrumentation |
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Concepts of
the Instrument |
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Practical I |
Discovering the
cytometer
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tour
of the
Instrument |
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Instrument Layout / Spectra site |
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Filters and Lasers exercise |
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Session III |
Fundamental Principals
I |
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Antibodies |
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Session IV |
Fundamental Principals
II |
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Fluorochromes |
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Session V |
Fundamental Principals
III |
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Compensation - |
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Practical II |
Compensation and Fluorchromes |
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Basics
of Compensation Worksheet |
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Instrument vs manual compensation demonstration |
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Session VI |
Fundamental Applications I |
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Immunology |
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End
Day 1 |
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Session VII |
Compensation |
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How
to do it correctly |
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Measurement Error |
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Hardware VS Software |
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Bi-exponential Scaling |
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Practical III |
Compensation Exercise |
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Understanding
the spread o f Data |
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Session VIII |
Cytometry Controls |
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Session IX |
Staining and Antibodies |
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The
process |
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Titration and Stain Index |
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Session X |
Fundamental Applications II |
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Cellular and DNA applications |
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Practical IV |
Staining Index and
Titration Exercises |
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Practical V |
Instrument
Demonstration |
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For Novices |
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Session XI |
Instrument
Characterization |
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For
the instrument and core managers |
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Session XII |
Fundamental Applications III |
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Bacteria, Plants and non mammalian flow |
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Session XIII |
Data Analysis Basics and Gating |
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End
Day 2 |
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Session XIV |
Fundamental Applications IV |
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Sorting and
Advanced Topics |
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Session XV |
Statistics |
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Practical VI |
Data Analysis and
Statistics Demonstration |
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Session XVI |
Optimizing
your experiments |
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with Controls
and characterization |
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Session XVII |
Strategies for Building |
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Design Process / Biological hypothesis |
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Antibody/Fluor combinations |
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Necessary, Important and Luxury Markers |
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instrument configurations |
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Practical VII |
Practice Designing Experiments |
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10 color experiment examples |
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Interactive Problem solving exercise |
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Session XVIII |
Presenting Good Data |
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Practical
VIII |
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and Practice
doing that |
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Session XIX |
Troubleshooting |
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What to do IF.... |
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Q&A |
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End Day 3 |
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Mario's Questions on Good Data |
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