Curriculum for this 1/2 day advanced flow course covers these topics:

I.          Introduction to Fluorescent Proteins

·         What are they,

·         A little history, how they were discovered

·         Why they are so useful.

·         How they are made, and how they are used in experiments.

·         Varieties currently available, and sources.

·         Recommendations of FPs for use in flow.

II.          Expressing Fluorescent Proteins for use in flow and image cytometry

·         Methods of transformation, transfection, and production of transgenotes.

·         Laser and detection configurations.

·         Compensation.

·         New lasers.

·         Subcellular targeting of FPs.

·         Stability and toxicity issues.

III.         Practical Examples of Analysis of FP Expression using Flow and Image Cytometry

·         Mammalian cells in culture.

·         Plant cells and organs.

·         Complex animal organs.  

·         Split FP reconstitution.

·         The “Brainbow”.

·         Kinetic FPs and nano-scale microscopy.

·         Noise in gene expression.