FloCyte has two possible programs for DNA studies, a Multiparameter DNA Cell Cycle Approaches Course, outlined below and the software training program for Modfit, from Verity.  Both will help your group become efficient in DNA Cell Cycle analysis.  Please contact FloCyte for more information on scheduling one or both of these courses. 

  Basics of Cell Cycle Analysis

             A very brief history of cell cycle

             Going from cell cycle to single parameter DNA histogram
             What do DNA histograms tell us – what other information is required for              interpretation?
             Brief discussion of DNA dyes – do’s and don’ts
             Examples of perturbations in DNA histograms
             Sub-G₁ cells – how to identify apoptotic cells from debris

  Multiparameter Cell Cycle

            BrdU to more precisely determine S phase

                        Traditional method

                        New SBIP method

            Other assays for proliferation – Ki-67, cyclins

            Early two parameter approaches

                        DNA versus protein

                        DNA versus RNA

            Native versus Denatured DNA for identification of mitosis
Use of cyclins to study cell cycle

            Background on role of cyclins, cdks and ckis

            Discussion of each of the useful cyclins: D, E, A and B

            Use of cyclins to study:

                        Refinement of cell cycle compartments

                        Drug treatment

                        Unbalanced growth

                        Drug point of action – stathmokinesis

                        Difference between cancer and normal cells

            Three parameter approaches using cyclins and DNA

            Multiparameter approaches using modification of chromatin proteins

            Phosphorylation of histone H3 to identify mitotic cells
            Phosphorylation of histone H2AX to identify DNA double-strand breaks

            Sensitivity of the assay

            Detection of DNA damage by a variety of clastogens

            Measuring DNA damage using ATM

            Other multiparameter approaches

Inactivated versus total retinoblastoma protein