Our Full day Curriculum covers these topics:

Introduction to the course

Physics of light

    Fluorescence

Fluorochromes and Reagents

    Considerations for selecting reagents

    Viability

Controls

    Beads for Instruments and assays

    Isotypes vs No isotypes

    FMOs

    Compensation Controls (more later)

Staining Methodology

    Titering and Saturation Issues 

    Autofluorescence Problems 

    Specific VS Non- specific fluorescence

Compensation

    In Multicolor phenotyping 

    How to do it correctly

    Measurement Error

    Hardware vs Software 

    Bi-Exponential Scaling

    Compensation Controls

Strategies for Building Multicolor Panels

    Antibody / Fluorochrome combinations

    Necessary, Important or Luxury Markers

    Calculating Spread and error

    Instrument configurations / filter choices

Practice Designing Experiments

    Design Process

    Biological hypothesis

For our Half Day Programs we concentrate on the Strategies for Building Multicolor Panels

And Issues Involved in designing them!