Curriculum for this 1/2 day advanced flow course covers these topics:

I.          General Introduction outlining the issues that need to be confronted.

II.          Problems associated with complex multicellular tissues.

·         Production of single cell suspensions, or

·         Production and analysis of homogenates.

III.         Dealing with large objects.

·         General introduction to sorting theory.

·         How to use conventional instruments with large particles.

·         Specialized instruments -- the Union Biometrica COPAS and BioSort.

IV.        Dealing with small objects.

·         Bacteria and smaller life forms, as analyzed using conventional sorters. 

·         Biosafety issues. 

V.         Analyzing homogenates and complex mixtures.

·         Plants.

·         Aquatic samples.

VI.        Case studies: working with model organisms.

·         Yeast.

·         Drosophila

·         Other insects

·         Your favorite organism (general discussion).

VII.       Case studies: Working with non-model and otherwise unusual organisms.