"Success in cytometry"

 The morning session will cover topics to help investigators understand how to achieve success in cytometry.  This will cover topics on the appropriate controls and how to use them; how calibration of instruments impacts experimental data; how to set appropriate voltages and understanding compensation.  Further material will cover basics on data presentation.

1. Controls

        a.  Compensation

        b.  Calibration

        C.  Controls

            i.   Experimental

            ii.  Instrumentation

            iii. Reference

 2. Setting good voltages

        a.  Application settings

        b.  Holden's method

 3.  Data presentation

The afternoon session, "Dots and plots and data ... Oh my." will discuss how principles of data analysis, such as the use of FMO's for setting gates, back gating and viability dyes.

Additional information on how to use statistics to validate data and test hypotheses.  A second lecture will talk about concepts on polychromatic panel design. 

1.  Data analysis and statistics

        a.  Gating

        b.  FMO controls

        c.  Statistics concepts

        d.  Statistical examples

2.  Designing good assays

3.  Fundamental polychromatic panel design

Please note: This training is not instrument specific, but applicable to any and all instruments.  We strive to teach you how to get the most out of your cytometer, not how to push the buttons. 

Typical training times are  9:00 AM to 5:00 PM each day for 1 full day.  This course is most appropriate for mixed level audiences, and will introduce concepts for novices but also cover topics of interest for anyone planning multi-color flow.