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Lecture 1: Flow Cytometric
Data
In this lecture, we will
discuss and review how data is generated on a flow cytometer, what that data
looks like and how it is stored. We will cover issues related to linear/log
transformation, binning vs channels
Lecture 2: The three C’s – Controls, Compensation and Calibration
This lecture will cover three major aspects of generating good
data, and sources of error in flow cytometry data. The proper use of controls
(both biological and gating), compensation and calibration of the instrument
are critical to understand to be able to generate good, reproducible data.
Lecture 3: Gating and Data Display
Having generated the data, this lecture will cover how to
display the data via uni- and bi-variant histogram so that you can perform
analysis. Practical aspects of gating your data using the proper controls
will be discussed.
Lecture 4: Statistics
With data properly analyzed, what can we get out of it? From
simple statistics, like percent of positive cells and mean fluorescent
intensity, this lecture will discuss the different types of statistics you can
get from flow cytometric data, and how to use them to answer your hypothesis.
Lecture 5: Reporting Your Data
This lecture brings all the previous lectures together. What
good is proper data analysis if it is not reported properly? This lecture
will cover how to present your data to the scientific community.
Lecture 6: The Future of Data Analysis
Where is flow cytometic analysis going? This lecture will give
a review of some of the exciting work on new methods of data analysis.
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